http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108192946-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-10 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5008 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0693 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 |
filingDate | 2018-01-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-108192946-B |
titleOfInvention | Method for screening human constitutive androstane receptor indirect activator |
abstract | The invention discloses a method for rapidly screening a human constitutive androstane receptor indirect activator, which comprises the following steps: diluting the I-type rat tail collagen to 100 mu g/mL by using an N-cyclohexyl-N' - (2-morpholinoethyl) cyanamide methyl p-toluenesulfonate aqueous solution with the concentration of 130 mu g/mL, and doping 20 mu g/mL of nano graphene serving as a sensitizer; then coating a 96-hole cell culture plate for later use; inoculating a human skin squamous carcinoma cell line A431 on a 96-well cell culture plate after coating treatment; and after 12h, replacing the culture medium containing the tested chemical, and carrying out conventional cell proliferation test and positive contrast detection to screen out the human constitutive androstane receptor indirect activator. The method is simple, rapid and low in cost, can be used for rapidly screening whether the chemical such as industrial raw material chemicals, micromolecular chemicals, pesticides and the like is a human constitutive androstane receptor indirect activator, and has wide application prospect. |
priorityDate | 2018-01-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 285.