http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108192893-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6895 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6895 |
| filingDate | 2017-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108192893-B |
| titleOfInvention | Method for developing blumea balsamifera SSR primer based on transcriptome sequencing |
| abstract | The invention provides a method for developing blumea balsamifera SSR primers based on transcriptome sequencing, which comprises the following steps: obtaining a set of blumea balsamifera genome transcription to form a sequence database; splicing the sequencing sequences into a transcriptome by using Trinity, and taking the longest transcript in each gene as a Unigene; bioinformatics analysis of Unigene sequences; adopting MISA1.0 to carry out SSR detection on the Unigene; primer3 is used for SSR Primer design and SSR Primer polymorphism identification. The invention successfully designs 17979 pairs of SSR primers, screens 30 pairs of primers related to the metabolic pathway of active ingredients, and verifies 9 parts of blumea balsamifera DNA from different origins, wherein the total number of polymorphic primers is 27, and the 27 pairs of SSR primers can be used for distinguishing blumea balsamifera plant materials from different geographical origins. The invention provides a new idea for the development of blumea balsamifera SSR primers. |
| priorityDate | 2017-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 22.