http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108192892-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1013 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate | 2018-03-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-108192892-B |
titleOfInvention | Kit for extracting RNA by hydroxyl nano-magnetic bead method and extraction method |
abstract | The invention discloses a hydroxyl nano magnetic bead RNA extraction kit and a method for quickly and briefly extracting RNA of fungus producing sclerotium fungi. The kit comprises a solution I, a solution II, a solution III, a solution IV, a washing solution, an eluent and a hydroxyl nano magnetic bead suspension. The polysaccharide substance of the fungus producing sclerotium is removed by the innovative use solution II, so that the interference of extracellular polysaccharide is avoided in the RNA extraction process. The hydroxyl nano magnetic beads used by the kit are also innovatively pretreated in the test process, RNA is used as a molecular target, the adsorption capacity of the hydroxyl nano magnetic beads on DNA and protein is reduced, and the operation has the outstanding characteristic of high specificity. The kit is suitable for RNA extraction of the fungus producing sclerotium, is simple and convenient to detect, and reduces interference of extracellular polysaccharide to RNA extraction. |
priorityDate | 2018-03-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 55.