http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108192868-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-599 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-2302 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0645 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0636 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0783 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0786 |
| filingDate | 2017-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108192868-B |
| titleOfInvention | The induced amplification method of immunocyte |
| abstract | The invention proposes a kind of induced amplification methods of immunocyte.This method comprises: mononuclearcell is inoculated into bioreactor and in 37 DEG C, 2~7%CO by (1) 2 , 8~10%O 2 And it is cultivated one day under aseptic condition, anti-human CD61 antibody is coated in the bioreactor in advance, it include the first culture medium in the bioreactor, first culture medium is the T cell amplification culture medium comprising 10% autologous plasma, 1000IU/ml IL-2 and 0.01KE/ml Sapylin；(2) culture will be continued 14~21 days by the mononuclearcell of step (1) culture processing, to obtain the immunocyte, wherein the 1st~8 day for continuing culture, is in the second culture medium and in 37 DEG C, 2~7%CO 2 , 8~10%O 2 And carried out under aseptic condition, second culture medium is the T cell amplification culture medium comprising 10% autologous plasma and 1000IU/ml IL-2 or the T cell amplification culture medium comprising 1000IU/ml IL-2；The 9th~21 day for continuing culture, is in third culture medium and in 37 DEG C, 12~18%O 2 , 2~7%CO 2 And under aseptic condition in, the third culture medium be the SuperCulture TM L500 culture medium comprising 1000IU/ml IL-2. |
| priorityDate | 2017-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 55.