http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108191981-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-605 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-26 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
filingDate | 2018-02-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-11-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-11-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-108191981-B |
titleOfInvention | A kind of preparation method of liraglutide intermediate polypeptide |
abstract | The invention belongs to the technical field of polypeptide preparation methods, and in particular relates to a preparation method of liraglutide intermediate polypeptide GLP-1 (7-37). The main steps include constructing recombinant liraglutide engineering bacteria, expressing the liraglutide intermediate fusion protein in the form of inclusion bodies through E. Polypeptide GLP-1(7-37). In this invention, by changing the signal peptide of the recombinant sequence, the expression mode is changed to the expression of intracellular insoluble inclusion bodies, and the expression amount is greatly increased; the inclusion bodies after washing are alkali-soluble without using a large amount of denaturing agent, and the protein concentration is 20-30g/L Add the inclusion body dissolution buffer at a high concentration of 2, and the renaturation time does not exceed 1h, and it can be digested with enzymes after dissolution; the process is reduced, the operation volume is reduced, the cost of reagents is reduced, and it is conducive to industrial scale-up; UniSP-50XS cation exchange separation and purification, High degree of separation. The purity of the liraglutide intermediate polypeptide prepared by the invention reaches more than 87%, and the yield is more than 85%. |
priorityDate | 2018-02-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 194.