http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108191934-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-535 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H17-08 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-535 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H17-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577 |
| filingDate | 2017-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-02-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-02-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108191934-B |
| titleOfInvention | Tildipirosin hapten derivative and preparation method and detection kit thereof |
| abstract | The invention relates to the field of immunology, in particular to a tildipirosin hapten derivative, a preparation method and a detection kit thereof, which specifically comprise: the method comprises the steps of firstly reacting trimethylchlorosilane (TMSCl) with tildipirosin to obtain tildipirosin protected by C-2' -OH TMS, then reacting with succinic anhydride to esterify C-3-OH, finally deprotecting to obtain a C-3 esterified product, namely a tildipirosin hapten derivative, coupling with carrier protein to complete synthesis of a complete antigen, taking the tildipirosin complete antigen as an artificial immunogen to immunize a mouse to obtain serum, taking the tildipirosin-VOA complete antigen as a coating antigen, and completing preparation of a monoclonal antibody, subclone screening, preparation of a tildipirosin enzyme linked immunosorbent assay kit and the like. The main reagents of the kit are provided in the form of working solution, and the detection method is convenient and easy to implement and has the characteristics of high specificity, high sensitivity, high precision, high accuracy and the like. |
| priorityDate | 2017-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 61.