| abstract |
The present invention provides a kind of sulfydryl derivatization L-PROLINE type organic-inorganic hybridization monolithic columns and preparation method thereof; its be by function monomer 1- (3- sulfydryl -2-D- methylacryloyl)-L-PROLINE, crosslinking agent methacrylate Polyhedral Oligomeric silsesquioxane, binary pore-foaming agent and initiator ultrasonic mixing it is uniform after; it is injected into the quartz capillary through silylating reagent; by " mercapto-alkene " click-reaction, thermal-initiated polymerization, the sulfydryl derivatization L-PROLINE type organic-inorganic hybridization monolithic column is made.Sulfydryl derivatization L-PROLINE type organic-inorganic hybridization monolithic column produced by the present invention has uniform column bed, high permeability and stronger chemical stability and mechanical stability, and the separation to the small organic molecules such as benzene homologues, polycyclic aromatic hydrocarbon, phenol, phenyl amines and protein bio macromolecular may be implemented. |