http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108181399-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-02 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 |
| filingDate | 2018-01-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-08-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-08-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108181399-B |
| titleOfInvention | The detection method of A2- beta-casein content in a kind of dairy products |
| abstract | The invention discloses a kind of detection methods of A2- beta-casein content in dairy products, including prepare sample and standard items: then water being added to be configured to lotion if it is milk powder, by lotion or cow's milk centrifugal degreasing；Buffer is added, is vortexed and mixes, stands 10-120min, is centrifuged 5-20min；Clear liquid is removed, buffer constant volume is added, is vortexed and mixes, is filtered with 0.45 μm of PTFE membrane filtration, to upper machine testing；HPLC is tested and analyzed, and is eluted using gradient elution program, mobile phase A: 0.1% trifluoroacetic acid, Mobile phase B: 0.1% acetonitrile.Determination method of the present invention may be implemented to analyze and research for A1- beta-casein in ordinary milk and A2- beta-casein hypotype state-detection, pass through pretreatment and the corrdinated adjustment of chromatographic condition, realize being sufficiently separated for two kinds of hypotypes, and then combined standard product carry out quickly judging whether cow's milk raw material belongs to sterling A2- beta-casein hypotype cow's milk and substitute existing GENE Assay analysis method, simplify the difficulty for testing and analyzing research, reduces and test and analyze cost. |
| priorityDate | 2018-01-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 61.