http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108181358-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-26 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-26 |
filingDate | 2017-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-02-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-02-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-108181358-B |
titleOfInvention | A method for the detection of amino acids based on Aeromonasin nanochannels |
abstract | The method for detecting amino acids based on aeromonasin nanochannels of the present invention comprises the following steps: (1) designing a DNA probe with an aldehyde group attached; (2) connecting the amino acid to the DNA probe; (3) preparing the aerolysin biological Nanochannel: ①Activation of Aeromonasin; ②Preparation of phospholipid n-decane solution; ③Preparation of phospholipid bilayer; ① Collect DNA probe single-molecule signals; ② Collect amino acid single-molecule signals; ③ Collect DNA probe-amino acid single-molecule signals; ④ Count the collected DNA probe-amino acid single-molecule signals to obtain amino acid characteristic information. Simultaneous detection of different amino acids. The invention can simultaneously detect different amino acids at the single-molecule level, and has the advantages of convenient operation, real-time accuracy, ultra-sensitive resolution, and very positive significance for life science research. |
priorityDate | 2017-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.