| abstract |
The invention discloses a method for detecting trace amounts of Fe 3+ , Hg 2+ and Cu 2+ in living cells by dual-channel fluorescence imaging. Triamineethylamine derivatives, referred to as probes, are used as dual-channel fluorescence imaging to detect active cells. Fluorescent imaging probes for medium and trace amounts of Fe 3+ , Hg 2+ , and Cu 2+ can detect trace amounts of Fe 3+ , Hg 2+ , and Cu 2+ in active cells through dual-channel fluorescence imaging; the method of the invention can realize the detection of multiple target ions detection, the probe in the present invention and Fe 3+ , Hg 2+ and Cu 2+ respectively form probe-Fe 3+ , probe-Hg 2+ , probe-Cu 2+ complexes in the cell, and emit Strong fluorescence at different wavelengths, convenient detection, good visibility, high sensitivity, good selectivity, and real-time online detection of Fe 3+ , Hg 2+ , Cu 2+ ions in cells. |