http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108165551-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-22 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2471 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01023 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2017-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108165551-B |
| titleOfInvention | Improved promoter, T vector composed of improved promoter and application of improved promoter |
| abstract | The invention belongs to the field of genetic engineering, and relates to an improved promoter and application thereof, wherein the improved promoter is obtained by mutating a nucleic acid sequence between a-35 region and a-10 region in a promoter region into an endonuclease recognition site. The invention can overcome the problem that the transcription or translation product of the exogenous gene initiated by the strong promoter of the vector adopting blue-white screening is possibly toxic to a host and can cause the cloning failure, can avoid the defect that the deletion of 1-2bp at the enzyme cutting site of the vector causes the gene frameshift mutation to generate false positive cloning, and can eliminate the false negative phenomenon that the plate is blue spots because the exogenous DNA fragment is small and the insertion of the exogenous DNA does not change the reading frame of the gene. |
| priorityDate | 2017-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 312.