http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108165545-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/H01F1-0054 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1013 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/H01F1-0045 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/H01F1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2018-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-04-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-04-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108165545-B |
| titleOfInvention | Preparation and application of silicon-based polypeptide-based biological nano magnetic beads |
| abstract | The invention discloses a preparation method of a biological nanometer magnetic bead based on silicon-based polypeptide, which comprises the following steps: s10, constructing a deletion mutant strain of the bacterial magnetic particle membrane protein gene mamC or mamF, namely a primary recombinant strain; s20, constructing a gene fusion expression vector of the silicon-based polypeptide and the bacterial magnetic particle membrane protein MamC or MamF; introducing the obtained expression vector into a primary recombinant strain, and screening a secondary recombinant strain for expressing the silicon-based polypeptide; s30, producing biological nanometer magnetic beads for expressing and displaying the silicon-based polypeptide through culturing and fermenting the secondary recombinant strains; s40, taking the biological nanometer magnetic beads displaying the silicon-based polypeptides as seed raw materials, and carrying out silicon-based modification and silicon oxide precipitation on the surfaces of the biological nanometer magnetic beads to obtain novel biological nanometer magnetic beads with silicon oxide shells and surface polypeptides; the silicon-based polypeptide is Silaffins polypeptide. |
| priorityDate | 2018-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 38.