http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108165516-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0016 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-125 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-06 |
filingDate | 2018-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-108165516-B |
titleOfInvention | Method for producing leucine dehydrogenase by fermenting bacillus subtilis |
abstract | The invention discloses a method for producing ammonia-acid dehydrogenase by fermenting bacillus subtilis, belonging to the field of enzyme engineering. According to the invention, through a strategy of adding different signal peptides to the N end of a target gene, a recombinant secretory expression plasmid is constructed and is expressed in a bacillus subtilis expression system, the effect of screening out PhoD signal peptides is optimal, and the secretory expression level of leucine dehydrogenase is improved and is 2.2 times that of a reference bacterium. In the method, the bacillus subtilis is a safe strain, and the recombinant protein is directly secreted to the outside of cells, so that the later-stage separation operation is simple, the method is more suitable for industrial application, and convenience is provided for the application of leucine dehydrogenase in the fields of medical diagnosis and the like. |
priorityDate | 2018-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 512.