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filingDate 2017-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2020-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2020-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-108159493-B
titleOfInvention Preparation method of alginate-hydrogel nanofiber scaffold
abstract The invention relates to a preparation method of an alginate-hydrogel nanofiber scaffold, which comprises the following steps: preparing a polycaprolactone solution; preparing a sodium alginate solution; adopting a double-nozzle electrostatic spinning system to carry out electrostatic spinning to obtain a polycaprolactone-sodium alginate nanofiber membrane; completely soaking the polycaprolactone-sodium alginate nanofiber membrane in a calcium chloride solution, fully washing with an ethanol water solution, freeze-drying, soaking and washing with chloroform, sequentially washing with ethanol and distilled water, and freeze-drying to obtain the alginate-hydrogel nanofiber scaffold. The alginate-hydrogel nanofiber scaffold has a stable three-dimensional structure and good biocompatibility, can be applied to 3D cell culture, and can optimize the penetration depth of cells.
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