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filingDate 2017-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2020-08-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2020-08-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-108107066-B
titleOfInvention SEM/ESEM cathode fluorescence imaging method for biological nano probe
abstract A SEM/ESEM-cathode fluorescence imaging method for biological nanoprobes belongs to the technical field of cathode fluorescence imaging, and particularly relates to the technical field of biological fluorescence imaging 2 -Au-Si composite film, Si, SiO 2 The method comprises the following steps of obtaining a CL image and a corresponding SE or TE electronic image and a composite image of the CL image and the electronic image by using an SEM external connection mode and a CL full light mode matched with a cathode fluorescence spectrum on any substrate including a carbon-based film, obtaining a single cell CL spectrum or a single cell CL single light image and each electronic image with a single wavelength by using an SEM fast scanning mode and a CL single light mode matched with a cathode fluorescence spectrum, thus obtaining a single cell and single molecule high-resolution photon image and a corresponding electronic image which are specifically marked by a nanoparticle/quantum dot probe, being capable of imaging at a low temperature of between room temperature and minus 180 ℃, being matched with fluorescent molecule/fluorescent protein probes with different luminescence characteristics, and reducing the irradiation damage of high-energy electron beams to a biological sample.
priorityDate 2017-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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