http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108103089-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 |
| filingDate | 2017-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108103089-B |
| titleOfInvention | A construction method of seamless multi-segment clone |
| abstract | The invention discloses a method for constructing seamless multi-segment clones. The carrier sequentially includes a promoter sequence, a ribosome binding site, a Golden Gate reaction site, a suicide gene, a Golden Gate reaction site, a terminator, a screening gene, a large intestine Bacillus replicons and encoded repressors. This scheme has the following advantages: the vector plasmid does not need to be linearized in advance, and the amount of plasmid is small, which can reduce the cost of plasmid extraction; the restriction endonuclease digestion reaction and the ligation reaction can be carried out in the same system; each reaction is Only one restriction enzyme is needed, and there is no need to consider the conflict of reaction conditions; during the reaction, the restriction enzyme site will be excised, and there is no need to consider whether the introduction of extra fragments will have adverse effects on the target gene; by artificially controlling the sticky ends, It can avoid background caused by self-ligation of cloning vector; seamless connection of up to 9 fragments can be performed. |
| priorityDate | 2017-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 16.