| abstract |
The present invention provides a kind of ultraviolet light receptor UVR8 genes of sweet potato and its cloning process.The ultraviolet light receptor UVR8 genes of sweet potato, as shown in SEQ.NO.4, cloning process comprises the following steps nucleotide sequence：(1) synthesis of the first chains of cDNA；(2) homologous clone of UVR8 conserved sequences；(3) amplification that UVR8 3 ' is held；(4) amplification that UVR8 5 ' is held；(5) sequence assembly.The species for being not required affiliation nearer in this way, as long as there is homologous gene, it is possible to the clone of core fragment is completed by designing multiple merger bases, this method is equally applicable to the gene cloning of other plants.And in being cloned at 5 ends, the phosphoric acid group of mRNA need not be removed with phosphorylase, again in ligase jointing primer, but according to M MuLV reverse transcription enzyme viabilities, in reverse transcription RNA at 3 ' ends plus 35 C, then it is paired plus 35 G to design prime end, completes process of reverse-transcription.This method is easy, saves time and cost, success rate is higher. |