http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108103037-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P33-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y103-99004 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P33-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2018-03-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108103037-B |
| titleOfInvention | A 3-sterone-Δ1-dehydrogenase mutant and its construction method |
| abstract | The invention provides a 3-sterone-Δ1-dehydrogenase mutant, which belongs to the technical field of genetic engineering and enzyme engineering; the amino acid sequence of the 3-sterone-Δ1-dehydrogenase mutant is as shown in SEQ ID NO. 2 shown. The construction method of the 3-sterone-Δ1-dehydrogenase mutant comprises the following steps: 1) connecting the 3-sterone-Δ1-dehydrogenase gene with an expression vector to obtain a wild-type recombinant vector; 2) to obtain a The wild-type recombinant vector is a template, and the 3-sterone-Δ1-dehydrogenase mutant recombinant vector is obtained by inverse PCR site-directed mutagenesis; 3) The obtained 3-sterone-Δ1-dehydrogenase mutant recombinant vector is transformed A recombinant strain is obtained from the expression strain, and the recombinant strain is cultured to obtain a 3-sterone-Δ1-dehydrogenase mutant. The specific enzyme activity of the 3-sterone-Δ1-dehydrogenase mutant was 85217.08 U/mg, which was 227.35% higher than that of the wild type. |
| priorityDate | 2018-03-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 326.