http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108102995-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y501-03 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-02 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-125 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-61 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-24 |
| filingDate | 2018-02-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108102995-B |
| titleOfInvention | D-psicose 3-epimerase production strain and immobilization method thereof |
| abstract | The invention discloses a D-psicose 3-epimerase production strain and an immobilization method thereof, belonging to the technical field of bioengineering. The recombinant bacillus subtilis pHY300PLK-DPEase for producing the D-psicose 3-epimerase is constructed, the recombinant bacillus is used as a production strain, the recovery rate of enzyme activity after the cell is immobilized reaches 64 percent, the optimum temperature of the immobilized cell is increased by 5 ℃ compared with that of the whole cell, and the optimum pH value is not obviously changed; the fructose with the concentration of 300g/L is used as a substrate to be continuously converted for 7 times, the conversion rate can still reach 28%, and the residual enzyme activity also reaches 81%. The immobilization method is simple and easy to implement, and the immobilized particles have excellent performance and high industrial application value. |
| priorityDate | 2018-02-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.