http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108101984-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-622 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-565 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K2039-505 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-18 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-395 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P19-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P19-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-18 |
| filingDate | 2017-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108101984-B |
| titleOfInvention | Preparation method and application of sclerostin single chain antibody |
| abstract | The preparation method and application of the sclerostin single-chain antibody, the preparation method of the patent of the present invention is as follows: extract the total RNA of the anti-SOST monoclonal antibody 5H3D1 hybridoma cells, and use RT-PCR method to amplify the variable region gene of the antibody light chain and the variable region gene of the heavy chain The variable region gene was introduced into Linker3 between the VL and VH genes by overlapping extension splicing PCR method, and connected into a single-chain antibody SOST‑scFv. The correctly sequenced scFv gene was cloned into the expression vector PET-22b(+) and then transferred into E.coliBL21 for soluble expression. The expression product was identified by polyacrylamide gel electrophoresis SDS-PAGE, and the reactivity of the expressed protein was detected by ELISA and West-blot . The patent of the present invention has the advantages of small molecular weight, high permeability, high antigen binding activity, significant promotion of bone formation and suppression of bone loss. |
| priorityDate | 2017-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 112.