http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108084255-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-22 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-4737 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-18 |
| filingDate | 2017-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108084255-B |
| titleOfInvention | Preparation of recombinant canine C-reactive protein and monoclonal antibody thereof |
| abstract | The invention belongs to the field of biotechnology. The invention provides a polypeptide, which comprises two dominant epitopes of canine C-reactive protein (CRP), and is coupled with KLH protein for enhancing the immune effect. The invention provides a canine CRP recombinant protein which mainly comprises two dominant antigen epitopes of canine CRP. In order to improve the yield of the recombinant protein in a prokaryotic expression system, the amino acid sequence of the recombinant protein is converted into a corresponding nucleotide sequence by adopting an escherichia coli preferred codon, and the nucleotide sequence is chemically synthesized to construct a recombinant protein expression vector. The invention also relates to the preparation of the recombinant protein monoclonal antibody, which is used for preparing monoclonal cell strains after antigen immunization, cell fusion and multiple rounds of screening, purifying the monoclonal antibody, respectively marking fluorescent microspheres, determining the optimal monoclonal antibody pairing combination through orthogonal experiments and being used for inflammation diagnosis. |
| priorityDate | 2017-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 89.