| abstract |
The invention belongs to field of natural organic chemistry, are related to a kind of method of the chlorogenic acid extracting from Cortex Eucommiae, and more particularly to a kind of using Enzymatic Extraction, high speed adverse current chromatogram isolates and purifies the new method of chlorogenic acid.Advantage of the present invention：Present invention extraction is quick, rapid, and products obtained therefrom purity is high, and environmental pollution is small.1st, using biological enzymolysis technology, it is fully dissolved out intracellular active ingredient.2nd, using high-speed countercurrent chromatography separation process is simple, solvent-oil ratio is few, the quick separating of active ingredient is realized in a short time, and purity is high.The present invention promotes active ingredient to be fully dissolved out, alcohol reflux extracts, then is isolated and purified using high speed adverse current chromatogram, and gained chlorogenic acid purity is high using biological enzyme. |