http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108070577-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-07007 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1252 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 |
| filingDate | 2018-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108070577-B |
| titleOfInvention | Antiserum interference TaqDNA polymerase and preparation and application thereof |
| abstract | The invention relates to an antiserum interference Taq DNA polymerase obtained by analyzing a hot spring metagenome, and relates to the antiserum interference Taq DNA polymerase and a preparation method and application thereof. The protein amino acid sequence of the Taq DNA polymerase for resisting serum interference is shown as SEQ ID No: 1 is shown in the specification; the nucleotide sequence of the gene of the Taq DNA polymerase for encoding the antiserum interference is shown as SEQ ID No: 2, respectively. The protein amino acid sequence of the enzyme has 13 amino acid mutations compared to the amino acid sequence of wild Taq DNA polymerase. The sequence is constructed, cloned and subjected to expression and purification of polymerase, the polymerase has higher polymerase activity than commercial Taq DNA polymerase and wild Taq DNA polymerase, the polymerase is used for detecting a simulated sample in serum, and a fluorescent quantitative PCR result shows that the polymerase has better tolerance than wild Taq DNA polymerase and commercial polymerase. Therefore, the enzyme can be used for direct PCR detection of serum samples without nucleic acid extraction. |
| priorityDate | 2018-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 417.