http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108060269-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-701 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2018-01-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108060269-B |
| titleOfInvention | DPO primer group for detecting porcine epidemic diarrhea virus, porcine transmissible gastroenteritis virus and porcine rotavirus and application thereof |
| abstract | The invention discloses a DPO primer group for detecting porcine epidemic diarrhea virus, porcine transmissible gastroenteritis virus and porcine rotavirus and application thereof. The invention designs a pair of DPO primers aiming at a TGEV-N gene, a PEDV-N gene and a PRoV-VP7 gene respectively, and establishes a multiple DPO real-time RT-PCR detection method for porcine transmissible gastroenteritis virus, porcine epidemic diarrhea virus and porcine rotavirus. The results show that the detection limit of the method is 5.3X 10 0 The target gene fragments can be efficiently amplified within the annealing temperature range of 40-65 ℃ by copies/mu L, which shows that the method has wide annealing temperature range, strong specificity of the DPO primer and no nonspecific amplification in the PCR reaction process. The multiple DPO real-time RT-PCR detection method disclosed by the invention is simple in design, strong in specificity and high in sensitivity, and provides a new technical means for quickly and accurately detecting three porcine viral diarrhea causing pathogens of TGEV, PEDV and PRoV. |
| priorityDate | 2018-01-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 60.