http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108060175-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2830-002 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-80 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-865 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-64 |
| filingDate | 2017-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-02-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-02-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108060175-B |
| titleOfInvention | Construction and application of inducible yeast transformation recombination system |
| abstract | The invention discloses a construction of an inducible yeast transformation recombination system and application thereof in large-fragment DNA capture. The iTAR vector contains P Gal1‑V10 、I‑SceI、Ble R CEN6, ARSH4, URA3, homology arm 1, homology arm 2 and I-SceI cleavage sites between the homology arms. The iTAR vector can induce a Gal1-V10 promoter through galactose to generate I-SceI enzyme, the I-SceI enzyme acts on an I-SceI enzyme cutting site, the iTAR vector is linearized, the large fragment DNA is transformed into saccharomyces cerevisiae containing the iTAR vector, degradation of an empty vector and homologous recombination in a yeast body are realized, and the purpose of cloning the large fragment DNA is achieved. The iTAR cloning method is simple and efficient, can quickly and accurately obtain the required large-fragment DNA from the genome without the restriction of enzyme cutting sites, and has the transformation efficiency of about 73 percent. |
| priorityDate | 2017-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 30.