http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108060170-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-50 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-125 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-57 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2017-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108060170-B |
| titleOfInvention | Novel metal ion-tolerant keratinase and application thereof |
| abstract | The invention provides a novel metal ion tolerant keratinase and application thereof, provides a keratinase excavated based on metagenome technology and an application method thereof, and belongs to the technical field of industrial biology. The whole length of the keratin enzyme gene is 1,149bp, and 382 amino acids are coded; bacillus subtilis WB600 was taken as an example to successfully achieve heterologous expression of the keratinase gene. The acquisition method of the keratinase gene is convenient and feasible, the gene is subjected to recombinant expression, the constructed recombinant strain can realize the secretory expression of the recombinant keratinase, and the recombinant keratinase has better tolerance to metal ions and surfactants and better enzyme stability. The recombinant strain has short fermentation period and is suitable for industrial production. In addition, the enzyme has good reducing capability, can be applied to the research of preparing nano silver particles by a biological method, and the prepared nano silver particles have good morphology and good bacteriostatic activity. |
| priorityDate | 2017-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 246.