http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108060159-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate | 2017-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2019-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2019-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-108060159-B |
titleOfInvention | A kind of DNA extraction method rich in polysaccharide polyphenol plant |
abstract | The invention belongs to technical field of molecular biology, and in particular to a kind of DNA extraction method rich in polysaccharide polyphenol plant.DNA extraction method provided by the invention rich in polysaccharide polyphenol plant is: being cracked using specific lysate to cell, and pass through Tris phenol-chloroform mixed liquor, chloroform and sodium perchlorate combination liquid carry out DNA extracting, the DNA of extracting is purified using homemade adsorption column film again, to obtain the genomic DNA of high quality.The DNA purity is high extracted using the DNA extraction method provided by the invention rich in polysaccharide polyphenol plant, impurity is low, it can also prevent the destruction of ultra-oxygen anion free radical or active oxygen to DNA simultaneously, the integrality for guaranteeing the genomic DNA extracted is conducive to the scientific researches such as construction of gene library, PCR analysis and Southern hybridization. |
priorityDate | 2017-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 62.