| abstract |
The invention discloses a kind of Chinese hamster ovary celI protein-free medium, including the following raw material：Alanine, arginine, aspartic acid, asparagine, cystine, cysteine, glutamine, glutamic acid, glycine, histidine etc., calcium chloride, magnesium sulfate, potassium chloride, potassium dihydrogen phosphate, disodium hydrogen phosphate, sodium acid carbonate, sodium hydroxide, green vitriol, Magnesium dichloride hexahydrate, copper sulphate, Sodium Pyruvate, manganous chloride, sodium selenite, ammonium molybdate etc., niacinamide, p-aminobenzoic acid, folic acid, ribose, uracil, lipoic acid, linoleic acid L, cholesterol, dexamethasone, estradiol progesterone, testosterone etc..Culture medium of the present invention has been entirely free of serum composition, has not contained animal and plant-derived albumen, included amino acid, vitamin, inorganic salts, organic matter, a small amount of hormone, is conducive to the purifying process of albumen, cell is without the adaptation process by subtracting serum；Cytoactive can be kept for a long time, extend the protein expression production time. |