http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108051550-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-80 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-00 |
filingDate | 2017-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-108051550-B |
titleOfInvention | Method for determining main acidic substances in cell culture medium |
abstract | The invention relates to a method for determining the main acidic substances in a cell culture medium, comprising the following steps: measuring the pH value of the cell culture medium before the culture is started, and recording the pH value as an A value; taking a culture medium from a bioreactor for culturing cells, measuring the pH value of the culture medium, and recording as a B value; shaking the cell culture medium to enable gas in the cell culture medium to overflow, and measuring the pH value of the culture medium after the gas overflows and recording the pH value as a C value; comparing the magnitude between the A value, B value and C value to determine the major species of the acidic substance in the cell culture medium. The method can quickly and accurately determine the type of the main acidic substance in the culture medium, is favorable for removing or neutralizing the acidic product in the culture medium in a subsequent targeted manner, and improves the efficiency and the cost performance. |
priorityDate | 2017-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 17.