| abstract |
The present invention relates to the assay methods of 3 unrighted acid EPA, DHA of ω in a kind of cod-liver oil, after this method performs the derivatization EPA, DHA in cod-liver oil using column front derivation, it is measured with gas chromatography, by correction up factor internal standard method calculate cod-liver oil in EPA, DHA content.The method of the present invention includes the following steps：1. cod-liver oil pre-column derivatization reaction prepares fatty acid methyl ester solution；2.EPA methyl esters, DHA methyl esters relative correction factor measure；3.EPA, DHA gas-chromatography assay；4. the content of EPA and DHA is calculated by correction up factor internal standard method. |