http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108048438-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-004 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y405-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2018-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-08-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-08-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108048438-B |
| titleOfInvention | Halohydrin dehalogenase mutant and application thereof |
| abstract | The invention discloses a halohydrin dehalogenase mutant and application thereof in synthesis of atorvastatin key intermediate (3R,5R) 6-nitrile-3, 5-tert-butyl dihydroxyhexanoate, wherein the halohydrin dehalogenase mutant Hhec-A has an amino acid sequence shown in SEQ ID No. 2. Compared with wild enzyme, the catalytic activity of the 1 halogenated alcohol dehalogenase mutant provided by the invention is improved by 22 times, and the specific activity is improved by about 20 times. When the mutant HheC-A is used for catalyzing the reaction process of converting D3 into A7 in a whole cell manner, when the input amount of D3 is 50g/L, the conversion rate of D3 is 92% in 9 hours, and the yield of A7 is 72%. The yield of the product of the mutant HheC-A catalyzing the conversion of D3 into A7 is 4.8 times that of the wild enzyme HheC. |
| priorityDate | 2018-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 261.