http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108047302-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-145 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 |
filingDate | 2018-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-08-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-08-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-108047302-B |
titleOfInvention | Extraction method of non-denatured protein and special extracting solution thereof |
abstract | The invention discloses a method for extracting non-denatured protein and a special extracting solution thereof. The extraction method comprises a step a 2): extracting non-denatured protein in a sample by using an extracting solution; the extract contains sucrose, NaCl, sodium L-ascorbate, digitonin, dithiothreitol, phenylmethylsulfonyl fluoride and protease inhibitor. The extraction method provided by the invention is simple and convenient to operate, has mild and economic reagents, can efficiently extract high-quality non-denatured protein, lays a foundation and a technical guarantee for the subsequent development of stress plant proteomics research, and has certain guiding significance for extracting the protein of the plant recalcitrant tissue living in the extreme environment. The invention has great application value. |
priorityDate | 2018-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 66.