http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108034667-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-66 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-145 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-242 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-66 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-57 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P1-02 |
| filingDate | 2018-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108034667-B |
| titleOfInvention | Monascus ruber alpha-amylase gene, and preparation method and application thereof |
| abstract | The invention provides a monascus ruber alpha-amylase gene, a preparation method and application thereof. The technical scheme is to screen an amylase protein expression gene which is highly homologous with aspergillus oryzae alpha-amylase A from the whole genome of monascus ruber NRRL1597, and a PCR amplification method is designed around the sequence characteristics of the amylase protein expression gene. On the basis, according to the invention, firstly, monascus ruber CICC41233 is taken as an experimental strain, a target gene is obtained through PCR amplification, then a binary plasmid expression vector pNeo0380-440333 is constructed through the target gene, and then the target gene is transformed into parent monascus ruber through mediation of agrobacterium tumefaciens EHA105, so that a monascus pigment high-yield strain is obtained, and the strain can remarkably promote degradation of rice starch, thereby improving the yield of monascus pigment. On the basis, the fermentation method special for producing the monascus pigment is designed according to the biological characteristics of the recombinant strain, the yield of the produced monascus pigment is obviously higher than that of a wild strain, and the proportion of alcohol-soluble components in the monascus pigment is increased. |
| priorityDate | 2018-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 362.