http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108034634-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0668 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0775 |
| filingDate | 2017-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108034634-B |
| titleOfInvention | Method for separating endometrial mesenchymal stem cells from menstrual blood |
| abstract | The invention provides a method for quickly and efficiently separating intrauterine membrane mesenchymal stem cells from menstrual blood, which comprises the following steps: collecting menstrual blood, centrifuging to remove upper plasma, adding the solution I into the sediment a, quickly mixing uniformly, standing for 1-3 minutes, adding the solution II, mixing uniformly, centrifuging, removing supernatant, suspending the obtained sediment b with a culture solution, inoculating the obtained sediment b into a cell culture bottle, changing the solution after 48 hours, removing non-adherent cells, changing the solution every 2-3 days, digesting and passaging when the solution is 90% confluent, and collecting cells of P3 generation, namely the purified EnMSCs. Compared with the traditional method, the method for preparing EnMSCs has no difference in purity, but the method has the advantages of short preparation time, no more than 15min of separation process, simple and controllable components of the used separation reagent, convenient operation, reduction in preparation cost and improvement in safety. |
| priorityDate | 2017-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
| Predicate | Subject |
|---|---|
| isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID5234 http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID448670727 |
Total number of triples: 13.