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filingDate 2017-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b7b6375d8a0403943478c34a9aa3cc44
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publicationDate 2018-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-108020622-A
titleOfInvention The method of liquiritin content in HPLC measure Chinese medicine removing jaundice lotions
abstract The invention discloses a kind of method that HPLC measures liquiritin content in Chinese medicine removing jaundice lotion, comprise the following steps:(1) reference substance solution is prepared:Reference substance solution is made with methanol in extracting liquorice glycosides reference substance;(2) testing sample solution is prepared:Precision takes Chinese medicine removing jaundice lotion sample to add methanol dilution, and testing sample solution is made;(3) reference substance solution injection high performance liquid chromatograph measure is taken, establishes liquiritin standard curve;(4) testing sample solution injection high performance liquid chromatograph measure is taken, the peak area of liquiritin is obtained, liquiritin content is obtained so as to be calculated according to standard curve;The step (3) and the chromatographic condition of (4):250mm × 4.6mm, 5 μm of Agilent TC C18 columns;Mobile phase:Methanol-water=30:70;Flow velocity 1.0mlmin ‑1 ;Wavelength switches:0~14 minute 238nm, 14~30 minutes 280nm, column temperature:30 DEG C, 2 μ l of sample size.Method provided by the invention is easy to operate, high efficiency and time conservation, and qualification result is objective and accurate.
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114689712-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114689712-B
priorityDate 2017-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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