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filingDate 2017-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2020-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2020-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-108020587-B
titleOfInvention Method for detecting staphylococcus aureus in milk with double signal amplification effects
abstract The invention discloses a method for detecting staphylococcus aureus in milk with double signal amplification effects, and belongs to the technical field of biosensing. The technology utilizes gold-platinum nano composite material and DNA mimic enzyme to synthesize H pair 2 O 2 The nano probe has good double catalysis effects, and the graphene/gold nanoparticle nano composite material is utilized to modify the electrode so as to improve the electron transfer capacity of the modified electrode, thereby realizing the rapid and high-sensitivity detection of staphylococcus aureus. Under the optimized experimental conditions, the electrochemical immunosensor shows a wider linear detection range for staphylococcus aureus. In addition, the sensor has been successfully used for quantitative detection of staphylococcus aureus in pure milk as well as yogurt samples. Compared with the traditional methods such as a plate bacterial colony counting method and the like, the method is convenient and quick, is simple to operate, has high sensitivity, and realizes the quick and high-sensitivity detection of the staphylococcus aureus.
priorityDate 2017-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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