http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108008128-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-531 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-532 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-558 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-532 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-531 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-558 |
| filingDate | 2017-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108008128-B |
| titleOfInvention | Kanamycin rapid detection test paper for nucleic acid aptamer recognition and functional magnetic microsphere separation pretreatment, and preparation and application thereof |
| abstract | A kanamycin rapid detection test paper for nucleic acid aptamer recognition and functionalized magnetic microsphere separation pretreatment, and preparation and application thereof belong to the field of environmental, food and medical analysis. The qualitative analysis of kanamycin is realized by observing the color development depth of a detection line by utilizing the good separation effect of the magnetic microspheres, the high affinity between the aptamer (Apt) and a target and the indicator mark characteristic of gold nanoparticles (AuNPs), and meanwhile, the quantitative analysis can be performed by utilizing a colloidal gold quantitative analyzer. The cDNA can mediate to form a sandwich structure to capture AuNPs in a detection line, the number of captured AuNPs is linearly related to the cDNA and further the concentration of kanamycin, and therefore kanamycin content can be judged according to the color development depth of the detection line. The test paper method for detecting kanamycin is simple, convenient and quick, the detection is not limited by environmental conditions, the result is visual, the detection process only needs 20 min, and the method is convenient to expand and apply. |
| priorityDate | 2017-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 55.