http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108007996-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-447 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6848 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-447 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate | 2017-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108007996-B |
| titleOfInvention | Identification of pearl vitality components |
| abstract | The invention discloses identification of pearl vitality components, which comprises the following specific steps: hydrolyzing Margarita to obtain water soluble Margarita protein, hydrolyzing the precipitate with acid to obtain acid soluble Margarita protein, centrifuging the precipitate, and freeze drying to obtain keratin; subjecting the keratin to enzymatic hydrolysis with peptide N-glycosidase F; carrying out electrophoresis on the pearl protein by using separation gel, dyeing the gel after electrophoresis, decoloring, scanning a map and analyzing; cutting off the developed protein gel, treating, performing enzymolysis with trypsin, and separating and extracting peptide fragments by liquid chromatography; and carrying out mass spectrum detection on the peptide fragments to obtain a mass spectrogram, measuring the molecular weight and the charge ratio m/z, and then carrying out database comparison by using the m/z value obtained by mass spectrum. The beneficial effects are that: the total protein components of the pearl obtained by the invention are complete, the hydrolysis of a protein sample is reduced, the active components of the pearl can be quickly, efficiently and accurately identified, and a theoretical basis is provided for further deep research and comprehensive development of the pearl protein. |
| priorityDate | 2017-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 67.