http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108004298-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_838354375423711d1f8239a99eed7c87 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-56 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2017-12-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_98323423e38505fa7dcfb1caefb18e30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1901c5d0f50604ce812ff0507babb0e4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a10faf0588ec327d2194e8ed4364d379 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a3f5178dfe2f99aab173937edfc17645 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_699ad479ae5c10c651cb3ec695aae295 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0aef48740653fc6fdc158c3430dd4bcf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_58e944f12648e7ca4c08c6d2a39eba48 |
| publicationDate | 2018-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108004298-A |
| titleOfInvention | Chemical-activated luciferase gene expression dog interferon alpha biological activity detection method |
| abstract | The invention discloses a kind of chemical-activated luciferase gene expression dog interferon alpha biological activity detection method and its application.This method comprises the following steps：The genetic fragment of the pMx1 of dog Mx1 albumen is obtained using PCR amplification；PMx1 luc fusion fragments are obtained by the 5' ends of the genetic fragment insertion pGL3 basic carrier luc genes of pMx1, then using PCR amplification；The genetic fragment of the pCMV and EGFP in pEGFP N1 carriers are substituted with pMx1 luc fusions fragment, builds pMx1 luc plasmids, transfectional cell, and select stable transfected cells strain；Colonized culture is carried out to the stable transfected cells strain filtered out；Standard curve is prepared with the dog interferon alpha standard items of gradient dilution, is incubated altogether in cell dog interferon alpha sample to be checked added after colonized culture, then fluorescence intensity, the potency of combined standard curve evaluation dog interferon alpha sample to be checked. |
| priorityDate | 2017-12-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 42.