http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108004275-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-01034 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0004 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1029 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-01035 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-44 |
filingDate | 2017-11-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-108004275-B |
titleOfInvention | Escherichia coli recombinant bacterium for producing adipic acid and application thereof |
abstract | The invention discloses an adipic acid-producing escherichia coli recombinant bacterium and application thereof, and belongs to the field of bioengineering. The invention uses colon bacillus BL21(DE3) as host, expresses beta-ketothiolase gene, 3-hydroxy acyl-coenzyme A dehydrogenase gene, 3-hydroxy adipoyl dehydrogenase gene, 5-carboxy-2-pentenyl coenzyme A reductase gene and adipoyl coenzyme A in a modularized over-expression way, and replaces promoter; the shake flask yield of the adipic acid can reach 2.23g/L, and the yield reaches 51.6%. The promoter of the recombinant strain is replaced without adding an inducer, so that the cost is saved. |
priorityDate | 2017-11-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 107.