http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108003243-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_03d3ff58566916c78c676d2c9fe1cd75 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-26 |
| filingDate | 2017-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4b7081e4b65d1bc6cccdfee81eb52be4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fa284c9940d60ec914515c6a5418a766 |
| publicationDate | 2018-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108003243-A |
| titleOfInvention | A kind of method of purifying protein |
| abstract | The present invention provides a kind of method of purifying protein, the above method includes the following steps:1) S100 protein families are selected to build the fusion protein expression carrier of albumen to be purified；2) expression of above-mentioned recombination fusion protein:Obtain expression thalline；3) above-mentioned recombination fusion protein isolates and purifies:Collect above-mentioned expression thalline, crush the cell of above-mentioned expression thalline, centrifuge and collect the supernatant containing above-mentioned recombination fusion protein, above-mentioned supernatant carries out anion-exchange column initial gross separation, by loading, elution and the eluate sample being blended in the range of 300 to 400 milliliters is collected, calcium chloride (CaCl is added into above-mentioned eluate sample 2 ), upper hydrophobic chromatography column, obtains the fusion protein after above-mentioned hydrophobic chromatography column purification；4) electroresis appraisal；5) digestion purifies the fusion protein after above-mentioned hydrophobic chromatography column purification.The method of new purifying protein proposed by the present invention, easy to operate, cost is low, and purification effect is good. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111733145-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112830967-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112830967-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109134621-A |
| priorityDate | 2017-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 271.