| abstract |
The present invention relates to the method for a kind of wet plus loose somatic embryo rescue, including (1) to gather wet plus pine-cones, obtain explant；(2) induction produces embryo callus；(3) propagation of embryo callus；(4) curing culture is obtained with the cotyledonary embryos for sprouting potentiality；(5) sprout culture and obtain the step of sprouting seedling；The collection is wet plus the time of pine-cones is July 2 June 20；Hormone composition in the inducing culture includes：2 2.4 μM of 6 benayl aminopurines, 0.008 0.012 μM of brassin lactones, the 2 of 7 7.4 μM, 4 D butyl esters.This method has inductivity, body embryo maturing rate and the germination rate of higher embryo callus. |