| abstract |
The invention discloses a method for detecting the Indel marker of beef cattle PLAG1 gene and a special kit thereof: based on PCR technology, using the blood genome DNA pool of beef cattle (Jixian Red Cattle) as a template, using specific primer pair P1 to amplify the bovine PLAG1 gene Indel site, then carry out agarose gel electrophoresis, and according to the electrophoresis result, different individuals are divided into deletion type, normal type and heterozygous type. The method provided by the invention is based on the correlation between the beef cattle PLAG1 gene Indel site and growth traits On the basis of sex, the method is not only simple, fast, and easy to be popularized and applied, but also beneficial to speed up the molecular marker-assisted selection breeding of beef cattle. |