| abstract |
The present invention is molecular diagnosis field, and a kind of molecular diagnosis method and reagent using urine Non-invasive detection prostate cancer and its Lymph Node Metastasis, includes the following steps：1. urine nucleic acid extraction and the reaction of purifying 2.DNA bisulfite conversions and the multiple 4. polygenes interpretations of result of gene promoter region of real time fluorescent PCR detection method of follow-up 3. methylation specific of purifying.Above-mentioned steps 1 and 2 can be merged into a step.Polygenes includes the promoter region of following gene：CRMP4, PITX2, RASSF1A, SOSTDC, CYBA, EFEMP1, KDM5D, GSTP1, WFDC2, TACSTD2 etc., the testing result of these genes, which is applied in combination, can obtain the diagnosis of prostate cancer and its Lymph Node Metastasis can be predicted. |