http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107988245-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01041 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2457 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-56 |
| filingDate | 2017-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107988245-B |
| titleOfInvention | Codon-optimized pullulanase, expression vector and construction method thereof |
| abstract | The invention discloses a codon-optimized pullulanase, an expression vector and a construction method thereof. It is prepared from bacillus subtilis Bacillus subtilis 168) Codon preference based on acid-tolerant pullulan strains ( Bacillus acidpullulyticus ) Pullulanase gene, base sequence design, and expression in bacillus subtilis by means of autonomous replicative plasmid and integrative plasmid. The invention also selects a promoter of the expression frame, an mRNA stabilizing sequence, a signal peptide and a combination thereof to obtain a combination which can efficiently secrete and express in the bacillus subtilis. And realizes industrial production and improves the stability of enzyme activity. |
| priorityDate | 2017-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 226.