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filingDate 2016-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ee4e1d77687546aead33c053962b8976
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publicationDate 2018-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-107980621-A
titleOfInvention A kind of tissue culture method after the long sub- low-temperature treatment of valve pocket orchid species
abstract The invention discloses a kind of tissue culture method after long sub- low-temperature treatment of valve pocket orchid species, comprise the following steps:(1) the long sub- low-temperature treatment of valve pocket orchid species;(2) long valve pocket orchid species is disinfected;(3) seed sprouts culture:Culture medium is sucrose+coconut palm breast+activated carbon+potato+agar powder, PH5.5, light culture 30 60 days, 25 ± 1 DEG C of cultivation temperature;(4) Protocorm:Culture medium is sucrose+potato+banana+activated carbon+agar powder, PH5.5, illumination cultivation 12hr/d, and intensity of illumination is 1500 1200LUX, 25 ± 1 DEG C of cultivation temperature;(5) culture of rootage:Culture medium is sucrose+potato+banana+activated carbon+agar powder, PH5.5, illumination cultivation 12hr/d, and illumination is 1500 1200LUX, 25 ± 1 DEG C of cultivation temperature;(6) test tube transplantation of seedlings.During the present invention carries out each stage of tissue culture after the blue progress low-temperature treatment of long valve pocket, employ suitable for the culture medium of long valve pocket orchid tissue culture, improve long valve pocket orchid percentage of seedgermination and seedling rooting rate.
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112931202-A
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priorityDate 2016-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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