| abstract |
The invention relates to a chitosan microsphere modified by α-butyruvonic acid grafted with polyethyleneimine and a preparation and application method thereof. The microspheres are chitosan microspheres (CS-PEI) grafted with polyethyleneimine on the surface, and the surface is modified with α-butyruvonic acid (KA). Wherein, the amount of polyethyleneimine grafted on the microspheres The anion exchange capacity is 918-1275 μmol/g, the amount of the modified α-butanuonic acid is 612-1860 μmol/g based on the cation exchange capacity, and the isoelectric point value of the microsphere is 5.5-10.0. The preparation process of the microsphere of the present invention is simple and the cost is low, and when applied to protein renaturation, it can assist protein renaturation and carry out adsorption and extraction of protein without polluting the renaturation system. |