http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107955835-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-118 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-106 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6886 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2017-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107955835-B |
| titleOfInvention | Primer pool and detection method for detecting BRCA1/2 gene mutation |
| abstract | The invention discloses a primer group and a method for detecting BRCA1/2 gene mutation. The primer group comprises three primer pools, 97 pairs of primers are provided, and the sequences of the upstream primer and the downstream primer are shown as SEQ ID No. 1-194 in sequence. Firstly, extracting DNA of a sample to be detected, performing multiplex PCR amplification by using the three primer pools, and then combining target fragments obtained by amplification of the three primer pools together to construct a library; finally, sequencing the library and analyzing biological information to obtain the BRCA1/2 gene mutation condition of the sample. The method has the advantages of high throughput, high accuracy, high sensitivity, high automation degree, less sample demand, low cost, rapidness, capability of detecting various mutation types, capability of simultaneously detecting multi-site mutation and the like, can be applied to high-risk screening, medication guidance, prognosis and the like of tumors, has important significance in the aspects of BRCA1/2 gene mutation detection and pathogenicity analysis and evaluation in China, and has wide application prospect. |
| priorityDate | 2017-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.