http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107955805-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y106-99003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0036 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-125 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-75 |
| filingDate | 2017-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107955805-B |
| titleOfInvention | NADH oxidase with improved stability and application thereof in acetoin production |
| abstract | The invention discloses an NADH oxidase with improved stability and application thereof in acetoin production, and belongs to the technical field of genetic engineering. The mutant of the invention mutates alanine at position 65 into cysteine on the basis of amino acid shown in SEQ ID N0.2. The mutant enzyme obtained by the invention has half-life (t) of 60 degrees 1/2 ) The mutation period is improved by 1.9 times. The invention shows that the 65 th amino acid residue is mutated into cysteine to form correct disulfide bond with 125 th cysteine residue of natural NADH oxidase, thereby improving the stability of the enzyme and strengthening the industrial application potential of the enzyme. |
| priorityDate | 2017-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 45.