abstract |
Provided herein are methods for producing conjugated immunoglobulins, the methods comprising: unblocking a cysteine at amino acid position 80 ("Cys80") in the light chain variable region of the immunoglobulin , wherein the immunoglobulin comprises a heavy chain variable region and a light chain variable region; and a thiol-reactive compound is conjugated to the Cys80, wherein the thiol-reactive compound comprises a thiol-reactive group. Also provided are antigen-binding molecules and methods for producing the antigen-binding molecules, immunoglobulins, and nucleic acid molecules encoding the immunoglobulins and host cells comprising the nucleic acid molecules, conjugated immunoglobulins, and use in Light chain variable regions in conjugated immunoglobulins. |